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2d clean up kit  (Cytiva Europe)


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    Cytiva Europe 2d clean up kit
    2d Clean Up Kit, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 95/100, based on 973 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/clean+2d/2-D+Clean-Up+kit/pmc13206807-217-9-12
    Average 95 stars, based on 973 article reviews
    2d clean up kit - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Purification:

    Article Title: Proteomic Analysis Comparing Effect of Feeding Practices on the Milk Fat Globule Membrane Proteins from Camelus dromedarius
    Article Snippet: .. The resulting soluble protein fraction was purified using a 2D Clean-Up Kit (GE Healthcare, Uppsala, Sweden), and the pH was adjusted to 8.5 with 100 mM NaOH. .. Total protein concentration was determined using the 2D-Quant Kit (GE Healthcare, Chicago, IL, USA).

    Article Title: A Novel Chitinase-Like Family of Candidate Effectors Unique to Aphids
    Article Snippet: Samples were concentrated using a Vivacell 250 Pressure Concentrator (Sartorius Mechatronics, UK) using a 5000 Da molecular weight cut-off (MWCO) polyethersulfone (PES) membrane, then Roche cOmpleteTM protease inhibitor cocktail (PIC) was added. .. After further concentration to approximately 250 μl using a Vivaspin 6 centrifuge concentrator (Sartorius Mechatronics, UK) with a 5000 Da MWCO PES membrane, samples were purified using a 2D Clean-up Kit (GE HealthCare) then protein pellets were re suspended in 25 μl 6 M urea, 2 M thiourea, 0.1 M Tris–HCl, pH 8.0. .. After reduction with dithiothreitol and alkylation with iodoacetamide, proteins were digested with Sequence Grade Trypsin (Promega) and ProteaseMax Surfactant Trypsin Enhancer (Promega) at 37°C for 18 h. Mass spectrometry and data analysis were done as previously described (Thorpe et al. 2024).

    Concentration Assay:

    Article Title: A Novel Chitinase-Like Family of Candidate Effectors Unique to Aphids
    Article Snippet: Samples were concentrated using a Vivacell 250 Pressure Concentrator (Sartorius Mechatronics, UK) using a 5000 Da molecular weight cut-off (MWCO) polyethersulfone (PES) membrane, then Roche cOmpleteTM protease inhibitor cocktail (PIC) was added. .. After further concentration to approximately 250 μl using a Vivaspin 6 centrifuge concentrator (Sartorius Mechatronics, UK) with a 5000 Da MWCO PES membrane, samples were purified using a 2D Clean-up Kit (GE HealthCare) then protein pellets were re suspended in 25 μl 6 M urea, 2 M thiourea, 0.1 M Tris–HCl, pH 8.0. .. After reduction with dithiothreitol and alkylation with iodoacetamide, proteins were digested with Sequence Grade Trypsin (Promega) and ProteaseMax Surfactant Trypsin Enhancer (Promega) at 37°C for 18 h. Mass spectrometry and data analysis were done as previously described (Thorpe et al. 2024).

    Membrane:

    Article Title: A Novel Chitinase-Like Family of Candidate Effectors Unique to Aphids
    Article Snippet: Samples were concentrated using a Vivacell 250 Pressure Concentrator (Sartorius Mechatronics, UK) using a 5000 Da molecular weight cut-off (MWCO) polyethersulfone (PES) membrane, then Roche cOmpleteTM protease inhibitor cocktail (PIC) was added. .. After further concentration to approximately 250 μl using a Vivaspin 6 centrifuge concentrator (Sartorius Mechatronics, UK) with a 5000 Da MWCO PES membrane, samples were purified using a 2D Clean-up Kit (GE HealthCare) then protein pellets were re suspended in 25 μl 6 M urea, 2 M thiourea, 0.1 M Tris–HCl, pH 8.0. .. After reduction with dithiothreitol and alkylation with iodoacetamide, proteins were digested with Sequence Grade Trypsin (Promega) and ProteaseMax Surfactant Trypsin Enhancer (Promega) at 37°C for 18 h. Mass spectrometry and data analysis were done as previously described (Thorpe et al. 2024).

    Clinical Proteomics:

    Article Title: Serum Protein Profiling of Patients at Risk to Develop Gastric Disease Based on a DSC Test
    Article Snippet: .. Plasma pools (S0 and S2) were treated with a 2D Clean-Up Kit (Cytiva, Uppsala, Sweden) and centrifuged. .. The resulting pellets were resuspended in lysis buffer (Thermo Fisher Scientific).

    Lysis:

    Article Title: Muscle Proteome Analysis of Facioscapulohumeral Dystrophy Patients Reveals a Metabolic Rewiring Promoting Oxidative/Reductive Stress Contributing to the Loss of Muscle Function
    Article Snippet: .. Proteins were selectively precipitated using the PlusOne 2D-Clean up Kit (GE Healthcare, Little Chalfont, UK), in order to remove non-protein impurities, and resuspended in lysis buffer. ..



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    a Schematic representation of phosphoproteomic workflow. PC-3 cells were differentially labeled with light ( 12 C) and heavy ( 13 C) Arg and Lys isotopes for 6 passages, then activated with clustered ephrin-A1-Fc (efnA1-Fc) or treated with clustered Fc control for 20 min. Following cell lysis and <t>clean-up,</t> equal amounts of lysates from each condition were combined and digested with LysC and trypsin. Phosphopeptides were enriched with TiO 2 and analyzed by LC-MS/MS. N = 4 independently SILAC-labeled, stimulated and processed biological replicates. The light and heavy amino acid labeling was alternated between replicates (“label swap”): replicates 1 and 3 were “forward labeled” (Fc control: light, ephrin-A1-Fc: heavy) and replicates 2 and 4 were “reverse labeled”. b Heat map showing the log2-transformed activated:control ratios across the four replicates for the 30 most regulated phosphopeptides. c Regulated phosphoproteins were categorized into protein classes according to function by manually curating information from HRPD, Panther and UniProt databases and the literature. GO enrichment analysis of Cellular Compartment ( d ) and Biological Process and Molecular Function terms ( e ). The ClueGo app for Cytoscape was used to calculate enrichment of terms associated with EphA2-regulated proteins compared to our own background dataset and to cluster related and redundant terms. Enrichment was evaluated using one-sided hypergeometric testing. FDR was controlled using the Benjamini–Hochberg correction for multiple hypothesis testing. Node size reflects the number of regulated phosphoproteins linked to a particular term; node color indicates the Benjamini–Hochberg corrected p value as a measure of significance as indicated.
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    a Schematic representation of phosphoproteomic workflow. PC-3 cells were differentially labeled with light ( 12 C) and heavy ( 13 C) Arg and Lys isotopes for 6 passages, then activated with clustered ephrin-A1-Fc (efnA1-Fc) or treated with clustered Fc control for 20 min. Following cell lysis and clean-up, equal amounts of lysates from each condition were combined and digested with LysC and trypsin. Phosphopeptides were enriched with TiO 2 and analyzed by LC-MS/MS. N = 4 independently SILAC-labeled, stimulated and processed biological replicates. The light and heavy amino acid labeling was alternated between replicates (“label swap”): replicates 1 and 3 were “forward labeled” (Fc control: light, ephrin-A1-Fc: heavy) and replicates 2 and 4 were “reverse labeled”. b Heat map showing the log2-transformed activated:control ratios across the four replicates for the 30 most regulated phosphopeptides. c Regulated phosphoproteins were categorized into protein classes according to function by manually curating information from HRPD, Panther and UniProt databases and the literature. GO enrichment analysis of Cellular Compartment ( d ) and Biological Process and Molecular Function terms ( e ). The ClueGo app for Cytoscape was used to calculate enrichment of terms associated with EphA2-regulated proteins compared to our own background dataset and to cluster related and redundant terms. Enrichment was evaluated using one-sided hypergeometric testing. FDR was controlled using the Benjamini–Hochberg correction for multiple hypothesis testing. Node size reflects the number of regulated phosphoproteins linked to a particular term; node color indicates the Benjamini–Hochberg corrected p value as a measure of significance as indicated.

    Journal: Oncogene

    Article Title: EphA2 regulates vascular permeability and prostate cancer metastasis via modulation of cell junction protein phosphorylation

    doi: 10.1038/s41388-024-03206-x

    Figure Lengend Snippet: a Schematic representation of phosphoproteomic workflow. PC-3 cells were differentially labeled with light ( 12 C) and heavy ( 13 C) Arg and Lys isotopes for 6 passages, then activated with clustered ephrin-A1-Fc (efnA1-Fc) or treated with clustered Fc control for 20 min. Following cell lysis and clean-up, equal amounts of lysates from each condition were combined and digested with LysC and trypsin. Phosphopeptides were enriched with TiO 2 and analyzed by LC-MS/MS. N = 4 independently SILAC-labeled, stimulated and processed biological replicates. The light and heavy amino acid labeling was alternated between replicates (“label swap”): replicates 1 and 3 were “forward labeled” (Fc control: light, ephrin-A1-Fc: heavy) and replicates 2 and 4 were “reverse labeled”. b Heat map showing the log2-transformed activated:control ratios across the four replicates for the 30 most regulated phosphopeptides. c Regulated phosphoproteins were categorized into protein classes according to function by manually curating information from HRPD, Panther and UniProt databases and the literature. GO enrichment analysis of Cellular Compartment ( d ) and Biological Process and Molecular Function terms ( e ). The ClueGo app for Cytoscape was used to calculate enrichment of terms associated with EphA2-regulated proteins compared to our own background dataset and to cluster related and redundant terms. Enrichment was evaluated using one-sided hypergeometric testing. FDR was controlled using the Benjamini–Hochberg correction for multiple hypothesis testing. Node size reflects the number of regulated phosphoproteins linked to a particular term; node color indicates the Benjamini–Hochberg corrected p value as a measure of significance as indicated.

    Article Snippet: Equal amounts (800 μg) of cell lysates from “heavy” and “light” conditions were pooled and proteins enriched using a 2D clean-up kit (GE Healthcare) and quantitated using the 2D Quant kit (GE Healthcare).

    Techniques: Labeling, Control, Lysis, Liquid Chromatography with Mass Spectroscopy, Multiplex sample analysis, Transformation Assay